The sterile 5 m M salicylic acid solution was sprayed onto PIS plantlets at room temperature.For wounding stress, two leaves (one at the top and one at the bottom) of each plantlet were wounded with a needle, 3–4 times along the blade.These proteins, classified as plant panallergens resistant to protease and heat treatment (Asero et al. 2007), are involved in plant defence and environmental stress response (Kader 1996, Arondel et al. So far, there have been few attempts to investigate or herbaceous crop species, which may be indicating the difficulties of molecular breeding and nucleic acid manipulation in trees.It is, therefore, tempting to find out whether expression of these two gene families in response to various stresses is regulated in parallel or independently, and whether expression levels vary in different tissues.Real Time q PCR data showed that both genes respond differently to various stresses.Furthermore, a high variation in transcription levels of both genes was observed in leaf tissues, while in roots both genes were expressed at a lower extent and down-regulated. In higher plants, unlike in animals, Ca Ms are present as multiple isoforms even within species.
Transcription levels of both genes were analysed in leaves and roots of micropropagated plantlets under low and high temperatures, salicylic acid and wounding stress, harvested after 0, 0.5, 1, 2, 4, 10, 24 and 72 h.
When shoots reached 3 cm in height, plants were transferred to a rooting medium (RM; DKV macro- and microelements with 0.65% agar and 0.5 mg IAA).
Total genomic DNA was extracted from 1 g of leaf tissue using DNeasy Plant Maxi Kit (Qiagen, Hilden, Germany) following the supplier’s instructions.
Calcium-binding proteins and pathogenesis-related (PR) proteins are crucial components of the inducible repertoire of plant stress and defence.
Considering the important role played by calmodulin (Ca M) and lipid transfer protein (LTP) in mediating plant signal transduction, the present study investigated the expression of (PIS) under various abiotic stress conditions.